Rat ApolipoProtein B100 (apo-B100) ELISA Kit

Cat.#:

JL11948

Regulatory Status:

RUO

Alternative:

Apo-B100

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

31.2-2000ng/mL

Sensitivity:

15.4ng/mL

Specificity:

This kit recognizes Rat apo-B100 in samples. No significant cross-reactivity or interference between Rat apo-B100 and analogues was observed.

Sample Type:

Serum;plasma;tissue homogenates;cell lysates;cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Cardiovascular Biology

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| apo-B100

Apolipoprotein B (ApoB) occurs in the plasma in two main isoforms, ApoB48 and ApoB100. Apolipoprotein B100 (apoB100) is absolutely required for VLDL assembly and secretion. The amount of VLDL TG secreted by the liver depends on the amount of TG loaded onto each lipoprotein particle, as well as the number of particles. Each VLDL has one apoB100 molecule, making its availability a key determinant of the number of VLDL particles, and hence TG, that can be secreted by hepatic cells.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Rat apo-B100 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	102
Cell Culture Supernates(n=8)	96-108	105

| Linearity

Samples were spiked with high concentrations of Rat apo-B100 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-96	90-110
1 : 2	Average (%)	91	93	96
1 : 4	Range (%)	89-102	87-108	105-115
1 : 4	Average (%)	94	98	108